Richard M. Kinney,Claire Y. H. Kinney,Duane J. Gubler,Siritorn Butrapet,Natth Bhamarapravati
申请号：
US13212845
公开号：
US08673316B2
申请日:
2011.08.18
申请国别(地区):
US
年份:
2014
代理人:
摘要：
Chimeric flaviviruses that are avirulent and immunogenic are provided. The chimeric viruses are constructed to contain amino acid mutations in the nonstructural viral proteins of a flavivirus. Chimeric viruses containing the attenuation-mutated nonstructural genes of the virus are used as a backbone into which the structural genes of a second flavivirus strain are inserted. These chimeric viruses elicit pronounced immunogenicity yet lack the accompanying clinical symptoms of viral disease. The attenuated chimeric viruses are effective as immunogens or vaccines and may be combined in a pharmaceutical composition to confer simultaneous immunity against several strains of pathogenic flaviviruses.
