A perineum overlay device for locating anatomical features within the perineum of a patient and/or needle insertion sites for targeting anatomical features within the perineum of a patient includes a section of flexible material and a plurality of markings printed on a first side of the section of the material. The section of flexible material is configured to conform and cover a portion of the perineum of a patient. The plurality of markings are each configured to identify a location of an anatomical feature within the perineum and/or a needle insertion site corresponding to a targeted anatomical feature within the perineum, when the section is positioned over the perineum of a patient.
