PROCESO DE PRODUCCIÓN DE UNA VACUNA RECOMBINANTE, DICHA VACUNA EXPRESABLE EN CEPAS DE ESCHERICHIA COLI Y SU USO PARA COMBATIR LA INFLUENZA A H1NI1 BROTE 2009.
INSTITUTO TECNOLOGICO Y DE ESTUDIOS SUPERIORES DE MONTERREY
发明人:
Gonzalo Ismael MENDOZA OCHOA,Mario Moisés ALVAREZ,José Manuel AGUILAR YAÑEZ,Sergio Ángel GARCÍA ECHAURI,Roberto PORTILLO LARA
申请号：
MX2009009565
公开号：
MX348663B
申请日:
2009.09.08
申请国别(地区):
MX
年份:
2017
代理人:
摘要：
The present invention particularly refers to a recombinant protein which is specifically recognized by antibodies in the serum of patients infected with Influenza A H1N1 outbreak 2009 and which is proposed as a vaccine or as a main active ingredient of a vaccine produced in Escherichia Coli against Influenza A H1N1 that caused the epidemic outbreak 2009. Said protein is a fragment of the Hemagglutinin protein of the Influenza A H1N1 outbreak 2009 expressed in Escherichia coli strains. Additionally, the invention presents and protects a process through which said main active ingredient for a recombinant vaccine against Influenza A H1N1 causing the epidemic outbreak 2009 is obtained and purified through E coli cultures. The proteins including in the structure thereof the amino acid sequence SEC ID No. 1 or SEC ID No. 2 or SEC ID No. 3 are particularly protected. The use of said proteins as vaccines or active ingredients of a vaccine against Influenza A H1N1 outbreak 2009 is also protected. The invention also protects the process through which the aforesaid protein is produced, including a suitable level of biological activity and at amounts and levels of purity that ensure the proposed process to be applied in the industry.La presente invención se refiere a una vacuna recombinante expresable en cepas de Escherichia coli caracterizada por contener la Sec ID No. 1 del fragmento no glicosilado de la hemaglutinina del virus de la Influenza A H1N1/2009, con un extremo N-terminal de 3 a 12 histidinas (Sec. ID No. 2 y la secuencia Sec ID No.3).
