CONSTRUCTION D'ACIDES NUCLEIQUES CONTENANT LE GENOME COMPLET DU VIRUS HUMAIN DE L'HEPATITE C, CELLULES REPLICATIVES DU GENOME COMPLET DU VIRUS RECOMBINE AYANT LA CONSTRUCTION D'ACIDES NUCLEIQUES TRANSFEREE DANS CELLE-CI ET PROCEDE DE PRODUCTION DE PARTICULE VIRALE DE L'HEPATITE C
The present invention provides a method for replicating efficiently an RNAcontaining fulllength HCV genomic sequence and a method for producing HCVvirus particlescontaining fulllength HCV replicon RNA or fulllength HCV genomic RNA by usinga cellculture system. Further, the present invention relates to a method forproducing hepatitis Cvirus particles which comprises culturing a cell, into which a replicon RNAcomprising anucleotide sequence comprising a fulllength genomic RNA sequence of hepatitisC virus ofthe genotype 2a, at least one selectable marker gene and/or at least onereporter gene and atleast one IRES sequence or the fulllength genomic RNA of hepatitis C virus ofthe genotype2a is introduced, and generating virus particles in the culture medium. Stillfurther thepresent invention relates also to a hepatitis C vaccine and an antibodyagainst hepatitis C virusparticles.
