An anti-inflammatory and regenerative formulation is disclosed. It is applied in a suitable dosage form to substantially heal Inflammatory Bowel Disease (IBD) in humans and animals. It comprises of probiotic microbial strains namely Lactobacillus acidophilus (A), Bifidobacterium bifidum (B), Streptococcus thermophilus (T) in the ratio of 1: 2: 1 in that order.