PRODUCTION, EXTRACTION AND PURIFICATION OF THE NOVEL POLYENE MACROLIDE CALLED PULITONE RESULTING FROM A HETERO-FERMENTATIVE PROCESS PERFORMED BY A YEAST AND THE FORMULATION THEREOF IN A BACTERICIDE PRODUCT FOR AGRICULTURAL USE.
ROSA LAURA ANDRADE MELCHOR,JOSE MANUEL NARVAEZ MASTACHE
申请号：
MX2012007377
公开号：
MX2012007377A
申请日:
2012.06.22
申请国别(地区):
MX
年份:
2013
代理人:
摘要：
The present invention describes the production, purification and biological effectiveness of a novel polyene macrolide, generically called Pulitone (1). The macrolide 1 is obtained from a sugar hetero-fermentative process performed by a mixture of 5 yeasts and a bacterium, during two discontinuous phases and in a static system. The polyene 1 is purified by liquid-liquid extractions in a discontinuous system, for obtaining a concentrate extract of the macrolide 1 (437 ppm). The molecular structure is established by the determination of the spectroscopic properties (RMN-1H anD 13C, COSY, HSQC, HMBC AND NOESBY) and spectrometric properties thereof /electrospray and FAB+). The in Vitro evaluations have pondered the biocide activity over some of the main phytopathogen bacteria that affect economical interest crops, of the cucurbitaceae, gramineous, solanacea, leguminous and brassicales families. A water-based liquid product was separated from concentrate 1, which is called Pulitone (r) and has established an efficient control over the incidence and damages produced by previously evaluated bacteria.La presente invención describe la producción, purificación y efectividad biológica de un nuevo macrólido polieno, denominado genéricamente Pulitona (1). EL macrólido 1 es obtenido a partir de un proceso heterofermentativo de azúcares por una mezcla de 5 levaduras y una bacteria, durante dos fases discontinuas y en un sistema estático. El polieno 1 se purifica mediante extracciones de líquido-líquido en un sistema discontínuo, para obtener un extracto concentrado del macrólido 1 (437 ppm). La estructura molecular se establece mediante la determinación de sus propiedades espectroscópicas (RMN-1H y 13C, COSY, HSQC, HMBC y NOESY) y espectrométricas (electrospray y FAB+). Las evaluaciones in Vitro han permitido ponderar la actividad biocida sobre algunas de las principales bacterias fitopatógenas que afectan cultivos de interés económico, de las familias Cucurbitáceas, Gramíneas, So
