An incubation method for improving oil production of Chlorella includes providing a Chlorella, incubating the Chlorella in an illuminated environment with an initial concentration OD682 value of 0.005 to 0.015. The incubation method for improving oil production of Chlorella is characterized in that an ultrasonic source having a strength of 1.24~2.75 mW/cm2 and a frequency of 0.5~5 MHz is applied to the Chlorella during incubation. The ultrasonic source is periodically applied with an applying time and a stop time equal to the applying time, and the applying time is 5 to 15 minutes.一種提升油脂產量的綠藻培養方法,包含:提供一綠藻;於20~30℃之溫度範圍下,於一光照環境中培養該綠藻,該綠藻之培養初始濃度OD682值係介於0.005~0.015之範圍;其特徵在於:於培養過程中,以一超音波源照射該綠藻,該超音波源之照射強度係介於1.24~2.75mW/cm2,照射頻率係介於0.5~5MHz,該超音波源係為週期性照射,具有等長之一超音波源照射時間與一超音波源停止時間,且該超音波源照射時間係介於5~15分鐘。
