METHOD OF OBTAINING RADIOLABELLED WITH RADIONUCLIDES ALPHA-FETOPROTEIN IODINE AND METHOD OF ITS IMPLEMENTATION FOR DIAGNOSTICS OF MALIGNANT TUMORS
- 专利权人:
- AVTONOMNAJA NEKOMMERCHESKAJA ORGANIZATSIJA "INSTITUT MOLEKULJARNOJ DIAGNOSTIKI" (ANO "INMODI")
- 发明人:
- SEVERIN EVGENIJ SERGEEVICH,KULAKOV VIKTOR NIKOLAEVICH,MOSKALEVA ELIZAVETA JUR'EVNA,KLIMOVA TAMARA PETROVNA,SLOBODJANIK IL'JA IGOREVICH,SEVERIN SERGEJ EVGEN'EVICH
- 申请号：
- RU20090109345
- 公开号：
- RU2404812(C1)
- 申请日:
- 2009.03.16
- 申请国别(地区):
- 俄罗斯
- 年份:
- 2010
- 代理人:
- 摘要：
FIELD: medicine. ^ SUBSTANCE: invention relates to medicine, particularly to oncology. To prepare alpha-fetoprotein labeled with radionuclides of iodine, a solution of alpha-fetoprotein is obtained placed in a vial. The vial is washed with radioactive iodine-123, iodine-125 (125I-"Éñ") or iodine-131 with phosphate buffer solution, which is then inserted into a vial with alpha-fetoprotein. As a result a mixture of iodine and alpha-fetoprotein is obtained. Solution of chloramine-B in phosphate buffer solution is added to the mixture. The mixture is then stirred for 2-5 sec, and sodium sulfite is added into it to stop the reaction with continued stirring for 50-70 sec. After that human serum albumin in phosphate buffer solution is added, and the mixture is coated to a column with Sephadex with subsequent union of fractions containing radioactive protein. For diagnosis of malignant neoplasm labeled with radionuclides of iodine alpha-fetoprotein is injected to a patient, and in a period from 5 to 24 hours aft
- 来源网站：
- 中国工程科技知识中心
