‘UFR-16’ is a new and distinct diploid citrus rootstock for improved disease resistance. ‘UFR-16’ has shown a positive reaction to the Huanglongbing disease (HLB, or citrus greening disease) in experimental field trials. Scion trees grafted on this rootstock initially show a reduced frequency of infection, and reduced disease symptoms once infected as compared to commercial diploid rootstocks. ‘UFR-16’ rootstock also showed tolerance to the Diaprepes/Phytophthora complex in greenhouse tests.