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GENETICALLY MODIFIED ANIMALS AND METHODS FOR PRODUCING THEM
专利权人:
РЕКОМБИНЕТИКС; ИНК. (US)
发明人:
КАРЛСОН Даниэль Ф. (US),ФАРЕНКРУГ Скотт К. (US)
申请号:
RU2015101740
公开号:
RU2015101740A
申请日:
2013.06.19
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
1. A method for producing a genetically modified animal, said method comprising exposing embryos or cells to mRNA encoding TALEN, wherein TALEN specifically binds to the target chromosome site in embryos or cells, cloning cells into a surrogate mother or implanting embryos into a surrogate mother, while the surrogate the mother, therefore, bears an animal that is genetically modified only at the target chromosome site for TALEN and does not contain the reporter gene. 2. The method of claim 1, comprising exposing the TALEN embryos without a reporter gene, wherein more than about 1% of the embryos comprise modification at the target chromosome site. The method according to claim 1 or 2, comprising obtaining embryos having a genetic structure that is known to be capable of expressing a set of traits, and acting on TALEN embryos without a reporter gene, and screening a born animal for modification and for expression of the set of traits. four. A method according to claim 1, comprising exposing the TALEN cells without a reporter gene and cloning the cells, with more than 1% of the cloned cells producing animals including a modification at the target chromosome site. The method of claim 1, comprising exposing the TALEN cells without a reporter gene, producing colonies of clonal cells, and testing a subpopulation of the members of the colonies to identify colonies that include modification at the target chromosome site. The method of claim 5, wherein testing a subpopulation of colony members is a destructive process. The method of claim 6, wherein the testing method is selected from the group consisting of nucleolytic analysis, sequencing, PAGE, PCR, primer extension, or1. Способ получения генетически модифицированного животного, причем указанный способ включаетвоздействие на эмбрионы или клетки мРНК, кодирующей TALEN, при этом TALEN специфически связывается с целевым хромосомным сайтом в эмбрионах или клетках,клонирование клеток в суррогатную мать или имплантацию эмбрионо
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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