A purified monoclonal antibody, or an antigen-binding portion thereof, which specifically binds to human clathrin heavy chain (CHC) is disclosed. The antibody, or antigen-binding portion thereof, exhibits at least one, two, three, four, five, six, seven, or all eight of the following properties: (a) specifically binds to pancreatic adenocarcinoma cells (b) binding to the cell surface and cytosol of cancer cells and tumor blood vessels (c) internalized by CHC-expressing cells (d) inhibiting tumor growth, invasion ability, migration, and angiogenesis (e) inducing apoptosis in cancer cells and human umbilical vein endothelial cells (f) inhibiting tumor growth and tumor blood vessels in pancreatic cancerin vivo (g) suppressing epidermal growth factor, transferrin, and VEGF internalizations by cancer cells and (h) suppressing hypoxia-inducible factor-1α expression and vascular endothelial growth factor secretion.本發明揭露一純化的單株抗體,或其抗原結合部分,其可專一性地結合至人類網格蛋白重鏈(clathrin heavy chain,CHC)。該抗體、或其抗原結合部分,表現至少一個、兩個、三個、四個、五個、六個、七個、或全部八個以下之特性:(a)專一結合至胰腺癌細胞;(b)結合至癌細胞及腫瘤血管之細胞表面及細胞溶質;(c)會被CHC表現細胞所內化;(d)抑制腫瘤生長、侵入能力、轉移及血管新生;(e)誘發癌細胞及人類臍靜脈內皮細胞之細胞凋亡;(f)活體內抑制於胰臟癌之腫瘤生長及腫瘤血管;(g)抑制癌細胞之表皮生長因子、轉鐵蛋白及VEGF的內化作用;以及(h)抑制缺氧誘發因子-1α之表現及血管內皮生長因子之分泌。
