A sterol composition in pumpkin seed oil and an application thereof, and a drug for treating benign prostatic hyperplasia are provided, which relate to a technical field of medicine. The sterol composition in the pumpkin seed oil includes components by weight percentage of: 0.2-2wt% cholesterol, 0.5-5wt% campesterol, 1.5-5wt% β-sitosterol, 15-40wt% stigmasterol, 20-40wt% ergosta-7,22-dien-3β-ol, 5-10wt% lanosterol, and 15-30wt% cholest-7-en-3β,5α-diol. The sterol composition in the pumpkin seed oil is a pumpkin phytosterol composition extracted from the pumpkin seed oil. Through combining the phytosterol components and controlling a proportion among the components, effects of alleviating and treating prostate diseases are achieved, especially for the benign prostatic hyperplasia.
