A sterol composition in pumpkin seed oil and an application thereof, and a drug for treating benign prostatic hyperplasia are provided, which relate to a technical field of medicine. The sterol composition in the pumpkin seed oil includes components by weight percentage of: 0.2-2 wt % cholesterol, 0.5-5 wt % campesterol, 1.5-5 wt % β-sitosterol, 15-40 wt % stigmasterol, 20-40 wt % ergosta-7,22-dien-3β-ol, 5-10 wt % lanosterol, and 15-30 wt % cholest-7-en-3β,5α-diol. The sterol composition in the pumpkin seed oil is a pumpkin phytosterol composition extracted from the pumpkin seed oil. Through combining the phytosterol components and controlling a proportion among the components, effects of alleviating and treating prostate diseases are achieved, especially for the benign prostatic hyperplasia.
