Plants have been the source of medicines in pharmacopoeia. Herbs have been utilized to treat acute and chronic disorders for thousands of years. Natural products, due to their easy availability, low cost and lack of side effects, make themselves attractive candidates for drug research. One such indigenous medicinal plant is Acafypha alnifolia Klein ex Willd, belonging to the family (Euphobiaceae) is used in the entire investigation. There is no systematic biological activity work that has been undertaken on this plant. The objective of the present study is to examine the antioxidant, Phytochemical assay and antibacterial activities of leaf extracts. In Tests for phytochemicals like total phenols, flavonoids and protein were positive in leaf extracts of Acafypha alnifolia.The free radical scavenging effect, Nitric oxide scavenging assay, Total antioxidant and the reducing power were analyzed in methanol and aqueous extracts of Acafypha alnifolia leaves. Both the extracts showed better antioxidant activity. The results of antibacterial activity of various extracts from the leaves of Acafypha alnifolia showed better inhibition towards Bacillus subtilis .Streptococcus pneumoniae, Escherichia coli, Salmonella typhi, Pseudomonas aeruginosa, Klebisella pneumonia, Proteus vulgaris and Staphycoccus aureus. This study is the first report of the antioxidant, phytochemical and antibacterial properties of Acafypha alnifolia. The leaf extracts can be used as an effective antioxidant, phytochemical and antibacterial agent to combat various ailments caused by the free radicals and the bacterial species.
