The present invention relates to a new crystalline form of 9-[(R)-2-[[(S)-[[[1-(isopropoxycarbonyl)-1-methyl]ethyl]amino]phenoxyphosphinyl]methoxy]propyl]adenine fumarate, and a preparation method and application therefor. Specifically, the present invention relates to a new crystalline form of 9-[(R)-2-[[(S)-[[[1-(isopropoxycarbonyl)-1-methyl]ethyl]amino]phenoxyphosphinyl]methoxy]propyl]adenine fumarate represented by formula (I), characterised in that the XRPD pattern of the crystalline form at least includes 2? ± 0.20o being: 5.08, 12.44, 13.18, 22.37, 23.37 and 28.56 diffraction peaks. The crystalline form of the present invention has features such as high bioavailability, significant efficacy, good stability, high yield and high purity, and contributes to the selection and design of a drug administration route and the determination of process parameters of a pharmaceutical preparation, thereby improving drug production quality.
