The present invention provides means and methods of transforming plant cells,seeds, tissues or whole plants in order to yield transformants capable ofexpressing all enzymes of the carotenoid biosynthesis pathway that areessential for the targeted host plant to accumulate carotenes and/orxanthophylls of interest. The present invention also provides DNA moleculesdesigned to be suitable for carrying out the method of the invention, andplasmids or vector systems comprising said molecules. Furthermore, the presentinvention provides transgenic plant cells, seeds, tissues and whole plantsthat display an improved nutritional quality and contain such DNA moleculesand/or that have been generated by use of the methods of the present invention.