您的位置: 首页 > 农业专利 > 详情页

COMPOSITIONS DESTINEES A L'INACTIVATION DE LA REPLICATION D'UN VIRUS ET LEURS PROCEDES DE FABRICATION ET D'UTILISATION
专利权人:
DUKE UNIVERSITY;EMORY UNIVERSITY
发明人:
CULLEN, BRYAN R.,KENNEDY, E. MATTHEW,BOGERD, HAL P.,SCHINAZI, RAYMOND F.,KORNEPATI, ANAND,MEFFERD, ADAM
申请号:
CA2940084
公开号:
CA2940084A1
申请日:
2015.02.18
申请国别(地区):
CA
年份:
2015
代理人:
摘要:
Provided herein are recombinant constructs, vectors and expression cassettes including a first promoter which is suitably a tRNA promoter operably connected to a first polynucleotide encoding a first single guide RNA and a second promoter operably connected to a second polynucleotide encoding a Cas9 polypeptide. The first single guide RNA includes a first portion complementary to a strand of a target sequence of a DNA virus and a second portion capable of interacting with the Cas9 polypeptide. Also provided are codon optimized Staphylococcus aureus derived Cas9 polynucleotides and polypeptides with nuclear localization signals and optionally an epitope tag. Also provided are constructs for production of sgRNAs including a tRNA. Methods of inhibiting viral replication, inhibiting expression of a target sequence from a virus or treating a viral infection or viral induced cancer using the compositions are also provided.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

意 见 箱

匿名:登录

个人用户登录

找回密码

第三方账号登录

忘记密码

个人用户注册

必须为有效邮箱
6~16位数字与字母组合
6~16位数字与字母组合
请输入正确的手机号码

信息补充