This invention provides a trivalent immunogenic composition including a soluble portion of a Mycoplasma hyopneumoniae (M.hyo) whole cell preparation; a porcine circovirus type 2 (PCV2) antigen; and a PRRS virus antigen, wherein the soluble portion of the M.hyo preparation is substantially free of both (i) IgG and (ii) immunocomplexes comprised of antigen bound to immunoglobulin.本發明提供一種三價免疫原性組合物,其包括豬肺炎黴漿菌(Mycoplasma hyopneumoniae,M.hyo)全細胞製劑之可溶部分、豬環狀病毒2型(PCV2)抗原及PRRS病毒抗原,其中該豬肺炎黴漿菌製劑之該可溶部分實質上不含(i)IgG與(ii)由結合至免疫球蛋白之抗原組成之免疫複合物二者。