Obshhestvo s ogranichennoj otvetstvennostyu firmy "Imtek"
发明人:
OSIDAK (RU),Осидак Егор Олегович (RU),DOMOGATSKIJ (RU),Домогатский Сергей Петрович (RU),Осидак Мария Сергеевна (RU)
申请号：
RU2019106356
公开号：
RU0002715715C1
申请日:
2019.03.06
申请国别(地区):
RU
年份:
2020
代理人:
摘要：
FIELD: medicine； pharmaceuticals.SUBSTANCE: invention refers to pharmaceutical industry, namely to a sterile transparent viscous-flow water-salt solution for making biomedical cell products or three-dimensional fabric engineering structures. Sterile transparent viscous fluid water-salt solution for making biomedical cell products or three-dimensional fabric engineering structures containing acid, purified collagen retains the ability to form fibrils and stable hydrogels in physiological conditions. Method of producing a collagen solution involves successive stages: 1) extraction of collagen-containing tissue under certain conditions； 2) multiple purification of acidic water-salt extract from potentially immunogenic macromolecules； 3) purification of acidic water-salt extract from remaining impurities of potentially immunogenic macromolecules； 4) purification from pyrogenic impurities； 5) purification from impurities of common and spore-forming microorganisms, as well as supramolecular aggregates of collagen； 6) bringing collagen solution to required concentration of collagen protein. Method of producing a collagen solution involves successive stages: 1) extraction of collagen-containing tissue in diluted organic acid solution； 2) multiple purification of acidic water-salt extract from potentially immunogenic macromolecules； 3) purification of acidic water-salt extract from remaining impurities of potentially immunogenic macromolecules； 4) purification from pyrogenic impurities； 5) purification from impurities of common and spore-forming microorganisms, as well as supramolecular aggregates of collagen； 6) bringing collagen solution to required concentration of collagen protein in it. Disclosed is use of collagen solution as initial material for formation of biopolymer matrix with inclusion of living cells； use of collagen solution for production of bioink for 3D printing with resolution of parts up to 0.3 mm and bioprinting of complex structures with inclusion of liv
