Leigh Burden,Steven D. Hume,Joshua Morton,Greg Pogue,Barry Bratcher,Hugh A. Haydon,Carrie A. Simpson,Nick Partain
申请号：
US16437734
公开号：
US20190374616A1
申请日:
2019.06.11
申请国别(地区):
US
年份:
2019
代理人:
摘要：
Disclosed herein are methods and exemplary compositions associated with virus purification, antigen purification, and conjugation of virus and proteins (e.g., antigen) to form vaccines for delivery of immunological and other therapeutic agents, exemplary aspects of which may include harvesting viral and antigenic substances from source organisms； a purification platform comprising chemical separation and size-difference separation for the removal of contaminants, debris and impurities from the viral and protein (e.g. antigenic, including influenza hemagglutinin antigens) substances, as well as their concentration and collection； and a conjugation platform providing activation of the virus at a pH that increases binding rate and binding propensity between the virus and the protein, wherein embodiments related to the conjugation platform include controlling the ratio of virus to protein.
