Lisa A. Scott-Carnell,Emilie J. Siochi,Nancy M. Holloway,Kam W. Leong,Karina Kulangara
申请号：
US12969076
公开号：
US09005604B2
申请日:
2010.12.15
申请国别(地区):
US
年份:
2015
代理人:
摘要：
A scaffold assembly and related methods of manufacturing and/or using the scaffold for stem cell culture and tissue engineering applications are disclosed which at least partially mimic a native biological environment by providing biochemical, topographical, mechanical and electrical cues by using an electroactive material. The assembly includes at least one layer of substantially aligned, electrospun polymer fiber having an operative connection for individual voltage application. A method of cell tissue engineering and/or stem cell differentiation uses the assembly seeded with a sample of cells suspended in cell culture media, incubates and applies voltage to one or more layers, and thus produces cells and/or a tissue construct. In another aspect, the invention provides a method of manufacturing the assembly including the steps of providing a first pre-electroded substrate surface electrospinning a first substantially aligned polymer fiber layer onto the first surface providing a second pre-electroded substrate surface electrospinning a second substantially aligned polymer fiber layer onto the second surface and, retaining together the layered surfaces with a clamp and/or an adhesive compound.
