SEBO, Lubomir,SHEN, Gene,YUE, Stephen,OSUNA, Honey,LAPIN, Yuri,BROGLEY, Louis,FEDOROV, Andrei
申请号：
WO2016US63178
公开号：
WO2017087975(A1)
申请日:
2016.11.21
申请国别(地区):
世界知识产权组织国际局
年份:
2017
代理人:
摘要：
Labeled nucleotide analogs comprising at least one avidin protein, at least one dye-labeled compound, and at least one nucleotide compound are provided. The analogs are useful in various fluorescence-based analytical methods, including the analysis of highly multiplexed optical reactions in large numbers at high densities, such as single molecule real time nucleic acid sequencing reactions. The analogs are detectable with high sensitivity at desirable wavelengths. They contain structural components that modulate the interactions of the analogs with DNA polymerase, thus decreasing photodamage and improving the kinetic and other properties of the analogs in sequencing reactions. Also provided are nucleotide and dye-labeled compounds of the subject analogs, as well as intermediates useful in the preparation of the compounds and analogs. Compositions comprising the compounds, methods of synthesis of the intermediates, compounds, and analogs, and mutant DNA polymerases are also provided.
