ROONEY, CLIONA M.,LEEN, ANN M.,VERA, JUAN F.,NGO, MINHTRAN V.,KNAUS, RAINER LUDWIG
申请号：
CA3025053
公开号：
CA3025053A1
申请日:
2012.04.23
申请国别(地区):
CA
年份:
2013
代理人:
摘要：
The present disclosure relates to a novel process for in vitro expansion ofautologous or allogenicantigen specific T cells comprising the steps: a) culturing a population ofautologous PCMB cellsin the presence of: i) dendritic cells which have been pulsed with apeptide/peptide mix relevantto a target antigen OR a peptide/peptide mix relevant to a target antigen, andii) at least onecytokine, and b) culturing a population of T cells from step a) in thepresence of: i) dendritic cellswhich have been pulsed with a peptide/peptide mix relevant to a target antigenOR autologousantigen presenting T cells (T-APCs) cells which have been pulsed with apeptide/peptide mixrelevant to a target antigen and an artificial co-stimulatory factor, and ii)optionally a cytokine, andcharacterized in that the process does not employ live virus and/or viralvectors or the use of DNAor RNA encoding antigens or recombinant target antigens in the expansion ofthe relevant T cellpopulation. The disclosure also extends to cell populations obtained from saidprocess,pharmaceutical compositions comprising the said cell populations and use ofthe cells andcompositions for treatment, particular the treatment or prophylaxis of virusinfection and/or cancer,for example in immune compromised or immune competent patients.
