Disclosed are a glycoprotein composition with low residual solvent level and preparation method thereof the organic solvent content of the glycoprotein composition is no more than 0.5% the method comprising the steps of: (a) vacuum drying the precipitate of a glycoprotein composition and an aqueous system together at 0 25�C (b) continue vacuum drying after removing the aqueous system and obtaining a glycoprotein composition with low residual solvent level.