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PROTEIN COMPLEXES OF CYTOMEGALOVIRUS
专利权人:
НОВАРТИС АГ (CH)
发明人:
КАРФИ Андреа (US),ВЭНЬ Инся (US)
申请号:
RU2015103891
公开号:
RU2015103891A
申请日:
2013.06.29
申请国别(地区):
RU
年份:
2016
代理人:
摘要:
1. A method for producing an isolated HCMV membrane protein complex containing gH, gL and at least one additional HCMV glycoprotein, wherein said method comprises recombinant expression of said gH, gL and at least one more HCMV glycoprotein. 2. A method for expressing an HCMV membrane protein complex comprising gH, gL and at least one more HCMV glycoprotein by: - introducing one or more recombinant nucleic acid molecules encoding gH, gL and at least one more HCMV glycoprotein into the expression system; - expressing said one or more nucleic acids in said expression system; and - purification of the specified complex of membrane proteins. 3. The method of claim 2, comprising the step of transfecting mammalian cells with a first nucleic acid construct encoding a gH fragment lacking a transmembrane domain, a second nucleic acid construct encoding a gL protein; and a third nucleic acid construct encoding at least one further HCMV.4 glycoprotein. The method according to any one of the preceding paragraphs, wherein the HCMV membrane protein complex consists of gH, gL and gO.5. The method according to claim 1 or claim 2, wherein the HCMV membrane protein complex consists of gH, gL, pUL128, pUL130 and pUL131A.6. The method of claim 5, wherein said: - pUL128 comprises or consists of any of the sequences set forth in SEQ ID NO: 13, 14, 15 or 33; - pUL130 contains any of the sequences set forth in SEQ ID NO: 16, 17 or 34, or consists of it; and / or pUL131A contains or consists of any of the sequences given in SEQ ID NO: 18, 19, 20 or 35. The method according to claim 1 or claim 2, wherein the HCMV membrane protein complex has a purity of>; 85%,>; 86%,>; 87%,>; 88%,>; 89%,>; 90%,>; 91%,>; 92%,>; 93%,>; 94% or>; 95% by weight. 8. The method according to claim 1 or claim 2, wherein one or more of gH, gL, gO, pUL128, pUL130 and pUL131A in the indicated complex of membrane proteins HCMV: have mammalian glycosylation character; and / or - do not have1. Способ
来源网站:
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来源网址:
http://www.ckcest.cn/home/

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