The present application discloses a simple process for the purification of neutral human milkoligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of acationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/orelectrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity.Contrary to the purification currently used in fermentative production of neutral HMOs, the presentedprocess allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filteredconcentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. petfood) applications.The present application discloses a simple process for the purification of neutral human milkoligosaccharides (HMOs) produced by microbial fermentation. The process uses a combination of acationic ion exchanger treatment, an anionic ion exchanger treatment, and a nanofiltration and/orelectrodialysis step, which allows efficient purification of large quantities of neutral HMOs at high purity.Contrary to the purification currently used in fermentative production of neutral HMOs, the presentedprocess allows the provision of HMOs without the need of a chromatographic separation. The so purified HMOs may be obtained in solid form by spray drying, as crystalline material or as sterile filteredconcentrate. The provided HMOs are free of proteins and recombinant material originating from the used recombinant microbial strains and thus very well-suited for use in food, medical food and feed (e.g. petfood) applications.
