John P. Gleeson,Tanya J. Levingstone,Fergal J. O'Brien,Fergal J. OBrien
申请号：
US14084164
公开号：
US09072815B2
申请日:
2013.11.19
申请国别(地区):
US
年份:
2015
代理人:
摘要：
The invention relates to a method for producing a multi-layer collagen scaffold. The method generally comprises the steps of: preparing a first suspension of collagen and freezing or lyophilising the suspension to provide a first layer； optionally preparing a further suspension of collagen and adding the further suspension onto the layer formed in the previous step to form a further layer, and freezing or lyophilising the layers, wherein when the layer formed in the previous step is formed by lyophilisation the lyophilised layer is re-hydrated prior to addition of the next layer； optionally, repeating the aforementioned step to form one or more further layers； and preparing a final suspension of collagen and pouring the final suspension onto the uppermost layer to form a final layer, and freeze-drying the layers to form the multi-layer collagen composite scaffold.
