A cell culture medium and system are provided which eliminate or at leastreduce the requirement for exogenous components such as serum and feedercells. The cell culture medium comprises an IGF and vitronectin or fibronectinand, optionally an IGFBP, and is particularly suitable for propagatingkeratinocytes for subsequent use in skin growth and regeneration. Thisinvention also relates to compositions and methods for skin growth andregenerationin situ, which utilize aerosol delivery of cultured keratinocytes.
