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METHOD OF CELL CULTURING AND CELL SHEET
专利权人:
HONDA, HIROYUKI;JAPAN TISSUE ENGINEERING CO., LTD;ITO, AKIRA;KOBAYASHI, TAKESHI;UEDA, MINORU;KAGAMI, HIDEAKI;HATA, KEN-ICHIRO;TERASAKI, HIROKO
发明人:
HONDA, HIROYUKI,ITO, AKIRA,KOBAYASHI, TAKESHI,UEDA, MINORU,KAGAMI, HIDEAKI,HATA, KEN-ICHIRO,TERASAKI, HIROKO
申请号:
WO2004JP02967
公开号:
WO2004083412(A1)
申请日:
2004.03.08
申请国别(地区):
世界知识产权组织国际局
年份:
2004
代理人:
摘要:
Epidermal keratinocytes not having magnetic fine particles incorporated therein and epidermal keratinocytes having magnetic fine particles incorporated therein are seeded in a 24-well ultralow adhesion plate. A neodymium magnet is disposed outside the well bottoms, and the cells are cultured for one day. As a result, control epidermal keratinocytes not having magnetic fine particles incorporated therein do not adhere to the well bottoms, resulting in non-formation of undifferentiated cell sheet. By contrast, epidermal keratinocytes having magnetic fine particles incorporated therein, while being attracted to the well bottoms by magnetic force, form a multilayered cell sheet. This cell sheet can be easily recovered by removing the neodymium magnet disposed outside the well bottoms, causing a magnet having a hydrophilic film stuck thereto to come close to the cell sheet from upside and attract it through the hydrophilic film, and updrawing them.
来源网站:
中国工程科技知识中心
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http://www.ckcest.cn/home/

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