WRIGHT TERRY R.,LIRA JUSTIN M.,WALSH TERENCE ANTHONY,MERLO DONALD J.,LIN GAOFENG,JAYAKUMAR PON SAMUEL
申请号：
NZ60818806
公开号：
NZ608188A
申请日:
2006.10.27
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Disclosed is a method of selecting for at least one transgenic plant cell comprising a polynucleotide that encodes an AAD-12 protein having an amino acid sequence selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 4, a variant of SEQ ID NO: 2, and a variant of SEQ ID NO: 4, said variant having aryloxyalkanoate dioxygenase activity and at least 95% sequence identity with an amino acid sequence selected from the group consisting of SEQ ID NO:2 and SEQ ID NO:4, wherein said method comprises applying an aryloxyalkanoate herbicide on and/or about said transgenic plant cells and to lacking plant cells that lack said polynucleotide. Further disclosed is a method of selecting for a transformed plant cell comprising a heterologous polynucleotide that encodes a protein having aryloxyalkanoate dioxygenase activity, wherein a nucleic acid molecule that encodes said protein hybridizes under stringent conditions with the full complement of a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, and SEQ ID NO: 5, wherein said method comprises subjecting a plurality of plant cells to transformation with said polynucleotide, then growing said cells in a concentration of a herbicide that permits transformed cells that express said polynucleotide to grow while killing or inhibiting the growth of cells that are not transformed with said polynucleotide, wherein said herbicide is selected from the group consisting of a phenoxy auxin and a pyridyloxy auxin herbicide. Further disclosed is a method of detecting whether a plant comprises a polynucleotide that encodes a protein having aryloxyalkanoate dioxygenase activity, wherein a nucleic acid molecule that encodes said protein hybridizes under stringent conditions with the full complement of a nucleic acid having a nucleotide sequence selected from the group consisting of SEQ ID NO: 1, SEQ ID NO: 3, and SEQ ID NO: 5, wherein said polynucleotide comprises a codon composition
