Provided are a non-conductive antibacterial sheet, a method for manufacturing thereof, and an antibacterial method, wherein it is enough that the feature height of the non-conductive antibacterial sheet is shallower than the conventional one, and hence many materials may be used for preparation thereof. The non-conductive antibacterial sheet of an acrylic resin comprises a micropatterned concave and convex surface in which there are produced concave and convex micropattern groups by forming multiple protrusion portions each having a nearly rectangular shape in a plane view on the surface thereof, wherein the protrusion portions are regularly formed in an x-direction and a y-direction orthogonal thereto with x-direction spaces and y-direction spaces, the both spaces having predetermined widths； a height from the surface of the sheet to the top of the protrusion portion is 0.4 μm to 1 μm； a bottom width of the protrusion portion is 2 μm to 3 μm, and the width of the x-direction space is equal to the bottom width of the protrusion portion； and the non-conductive antibacterial sheet exhibits an antibacterial effect in such a way that bacteria which are in static contact with the micropatterned surface are not trapped in the x-direction spaces.
