An environmentally- and user-friendly, aqueous antimicrobial solutioncomprising effective amounts of hydrogenperoxide, glycolic acid, and at least one antimicrobially-active solventchosen from benzyl alcohol and phenoxyethanol; concentratedversions of same; kits that can be used to make same; wipes containing same;and the use of same in a method of antimicrobialtreatment of a surface contaminated with a microorganism (e.g. bacterial orfungal spore such as C. Difficile).