MIEDZYNARODOWY INSTYTUT BIOLOGII MOLEKULARNEJ I KOMORKOWEJ
发明人:
Janusz Marek Bujnicki,Agata Agnieszka Sulej,Krzysztof Jerzy Skowronek,Marcin Nowotny
申请号：
US14095351
公开号：
US09353358B2
申请日:
2013.12.03
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The subject of the invention is the ribonuclease which cleaves RNA strand in DNA-RNA hybrids, wherein ribonuclease comprises fusion protein comprising catalytic domain of RNase HI (RNase HI) or derivative thereof with a zinc finger DNA-RNA hybrid binding domain, and wherein the zinc finger binding domain has the ability to bind to specific sequences in the DNA-RNA hybrid. The invention also relates to new methods for determination of the sequence preference of DNA-RNA hybrid binding protein(s) or its domain(s) and allows determining the sequence recognized by sequence specific binding protein in the DNA-RNA hybrid.
