Provided are a biological sample transfer device and biological sample transfer method less likely to adversely affect a biological sample transferred from a biological sample collection vessel to a biological sample storage vessel. The biological sample transfer device (1) is provided with: a biological sample collection vessel (2) in which a biological sample is collected a biological sample storage vessel (3) composed of a flexible member, the biological sample storage vessel (3) having a biological sample accommodation chamber (311) formed by three-dimensional molding a connecting tube (4) that connects the biological sample collection vessel (2) and the biological sample storage vessel (3) in an aseptic and air-tight manner a port section (5) which is provided to the connecting tube (4) and by which a gas in the interior of the connecting tube (4) can be guided to the exterior while maintaining the air-tight state of the biological sample collection vessel (2), the biological sample storage vessel (3), and the connecting tube (4) and an upstream closure (6) provided closer to the biological sample collection vessel (2) side than the port section (5).
