A method for making proteins biologically active is suitable for activating a protein-containing sample, and sequentially includes a pretreatment step and an activation step. In the pretreatment step, the sample is irradiated with a heating source capable of emitting heat-maintaining light, and the sample is stirred so that the temperature of the sample reaches between 40°C and 42°C. In the activation step, the sample is irradiated with a light source with a wavelength of 420 nm to 460 nm. Through the activation step, the protein in the sample is activated into active protein to facilitate digestion and absorption.一種使蛋白質具有生物活性的方法，適用於活化一含蛋白質之樣品，並依序包含一預處理步驟，及一活化步驟。在該預處理步驟中，以一能發射維持熱度的光之加熱源照射該樣品，並攪拌該樣品，使該樣品的溫度達40℃至42℃之間。在該活化步驟中，以一波長為420奈米至460奈米的光源照射該樣品。藉由該活化步驟將該樣品中的蛋白質活化成具有活性之蛋白質，以利於消化並吸收。
