A fertility restorer gene in wheat and use thereof, belonging to the field of plant biotechnology； specifically relating to a clone of a recessive nuclear male sterility gene and a promoter thereof, and the use thereof in cross breeding. A fertility restorer gene FRG1 was successfully cloned by means of flow cytometry and high-throughput sequencing. The gene can completely restore the male fertility of Lanzhou nuclear male sterile mutant or an allelic mutant thereof, laying a foundation for a new cross breeding technology system in wheat. In addition, more possibilities are provided for solving the technical bottleneck problems, such as unstable fertility of sterile lines, limited resources for crossbred cultivars, the complicated technology for seed production, the high cost of seed production and the like, which are encountered within "three lines" and "two lines" hybridization technology in wheat. The provided method for propagation and maintenance of the gene and sterile line has important significance and application value for hybrid breeding in wheat.
