A method of purifying triterpenoid-based compound is described. A crude extract of Ganoderma is provided, wherein the crude extract of Ganoderma includes a first-type triterpenoid-based compound and a second-type triterpenoid-based compound. The first-type triterpenoid-based compound includes ganoderic acid A. The second-type triterpenoid-based compound includes ganoderic acid F and ganoderiol B. Next, simulated moving bed chromatography was used to separate triterpenoid-based compound from the crude extract of Ganoderma. By using the simulated moving bed chromatography, the first-type triterpenoid-based compound including ganoderic acid A may be separated from the second-type triterpenoid-based compound including ganoderic acid F and ganoderiol B in the crude extract of Ganoderma, hence forming triterpenoid-based compound with high purity.一種純化三萜類化合物的方法，包括提供靈芝粗萃物，所述靈芝粗萃物包括第一類三萜類化合物以及第二類三萜類化合物。第一類三萜類化合物包括靈芝酸A，第二類三萜類化合物包括靈芝酸F以及靈芝醇B。接著，以模擬移動床層析法將所述靈芝粗萃物中的三萜類化合物分離開來。運用此模擬移動床層析法可將靈芝粗萃物中的包括靈芝酸A的第一類三萜類化合物與包括靈芝酸F以及靈芝醇B的第二類三萜類化合物分離開來，以產生高純度的三萜類化合物。
