Innovative non-surgical methods and compositions are provided for administering PDT to promote tissue regeneration or augmentation while minimizing scarring and risk of infection. Among several areas of application, is the treatment of acute and chronic wounds which have afflicted epidermal and connective tissue layers of the body. Another application area is as cosmetic surgery/treatments, including: reducing wrinkles, sulcus, scars (acne or traumatic caused), sequelae cellulite, as well as for other skin irregularities, to give a smoother skin surface. This invention consists of a collagen based or other suitable biodegradable supporting matrix which is embedded with a liposomal loaded photosensitizer. In on embodiment of this invention a liposomal formulated photosensitizer is first injected at the site followed by collagen implantation and PDT treatment. In another embodiment a liposomal formulated photosensitizer is incorporated in the collagen. Generally 30 minutes after collagen is mixed with photosensitizer the light activation is done. The matrix may also carry important growth factors and cytokines, which promote fibroblast cell migration and proliferation, to the wound site. Microbial infection at the wound site can also be controlled by antibacterial PDT.創新非外科手術方法及組成物係被提供用於給予PDT,以促進組織再生或增大,而最低化疤痕及感染風險。在幾個應用領域之中係處理急性及慢性傷口,其具有身體之已被折磨的表皮及結締組織層。另一應用領域係用作美容外科手術/處理,包括:減少皺紋、溝、疤痕(痤瘡或創傷所造成)、橘皮組織後遺症,以及用於其他皮膚異常,以給定較平滑的皮膚表面。本發明由基於膠原蛋白或其他適合的生物可分解之支撐基質(其係包埋有裝載於微脂粒之光敏物)所組成。在本發明之一個具體實例中,微脂粒調配之光敏物係首先被注射到該部位,接著膠原蛋白植入及PDT處理。在另一個實例中,微脂粒調配之光敏物係被納入膠原蛋白中。一般而言,在膠原蛋白與光敏物混合後30分鐘,光活化被完成。基質也可以攜帶重要的生長因子及細胞介素(其促進纖維母細胞遷移及增生)到傷口部位。在傷口部位之微生物感染也可以藉由抗菌PDT被控制。
