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POLYETHYLENE GLYCOL / PEPTIDE CONNUGATES CONNECTED WITH A DISULPHID BOND FOR TRANSFECTION OF NUCLEIC ACIDS
专利权人:
КУРЕВАК ГМБХ (DE)
发明人:
БАУМХОФ Патрик (DE),ШЛАКЕ Томас (DE)
申请号:
RU2012112640/04
公开号:
RU2012112640A
申请日:
2010.09.03
申请国别(地区):
RU
年份:
2013
代理人:
摘要:
1. The molecule of the polymer carrier of the formula (I): LPS- [SPS] -SPL, in which the components P and P are different or identical to each other and represent a linear or branched chain hydrophilic polymer, each of the components P and P represents at least one -SH- the part of the molecule capable of forming a disulfide bond upon condensation with component P, a linear or branched chain hydrophilic polymer selected independently from each other from polyethylene glycol (PEG), poly-N- (2-hydroxypropyl) methacrylamide, poly-2- (methacryloyloxy) ethylphospho ilcholines, poly (hydroxyalkyl-L-asparagine), poly (2- (methacryloyloxy) ethylphosphorylcholine), hydroxyethyl starch or poly (hydroxyalkyl-L-glutamine), wherein the hydrophilic polymer chain has a molecular weight of from about 1 kDa to about 100 kDa. Cationic or a polycationic peptide or protein with a length of from about 3 to about 100 amino acids, or a cationic or polycationic polymer with a molecular weight of from about 0.5 kDa to about 30 kDa, each P represents at least two -SH moieties capable of forming a disul f-bond when condensed with additional Riley components, Ri / or P component (s); -SS- is a (reversible) disulfide bond; L is an optional ligand that can be present or absent, and can be selected independently from other components from RGD, transferrin, folate, signal peptide or signal sequence, signal or sequence of nuclear localization, penetrating the cell peptide (PKP), TAT, receptor ligand, cytokines, hormones, growth factors, low molecular weight compounds1. Молекула полимерного носителя формулы (I):L-P-S-[S-P-S]-S-P-L,в которойкомпоненты Pи Pразличны или идентичны по отношению друг к другу и представляют линейную или разветвленную цепь гидрофильного полимера, каждый из компонентов Pи Pпредоставляет по меньшей мере одну -SH-часть молекулы, способную формировать дисульфидную связь при конденсации с компонентом Р, линейную или разветвленную цепь гидрофильного полимера, выбранную независимо
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