Uses of a bacteriostatic agent formula in preparing a composition for vaginal use. The bacteriostatic agent formula provides enhanced inhibitory effects with respect to Escherichia coli and/or Staphylococcus aureus and/or Candida. Also related is the composition for vaginal use that provides enhanced inhibitory effects with respect to Escherichia coli and/or Staphylococcus aureus and/or Candida, promotes restoration of beneficial Lactobacillus and enhances vaginal acidity when used in the vagina, and is applicable in the regulation of vaginal micro-ecology, regulation of vaginal flora, and selective vaginal decontamination.