This invention relates to a chalaza hydrolysate, a preparation method and a use thereof. The preparation method comprises the steps of hydrolyzing a chalaza material by an enzyme to obtain a hydrolysate solution; filtrating and lyophilizing the hydrolysate solution to obtain a chalaza hydrolysate which comprises leucine, arginine, phenylalanine, valine and lysine. Thus, the chalaza hydrolysate has effects of reducing lipid and oxidative stress of liver, and can be used for manufacturing compositions for liver protection.本發明係一種卵繫帶水解物及其製備方法與用途,製備方法係以酵素水解一卵繫帶材料後獲得一水解液,過濾水解液並凍乾後獲得一卵繫帶水解物,係包含白胺酸、精氨酸、苯丙胺酸、纈胺酸與離胺酸;卵繫帶水解物具備降低肝臟脂肪堆積與降低肝臟氧化壓力之功效,藉此可用於製備保護肝臟之組成物。