The invention relates to a method of tumoral vascularization detection in a test substrate comprising (i) determining (150) at least one parameter from the modeling performed according to a method for processing a test digital signal when resulting from the excitation of a contrast agent present in said test substrate wherein the at least one parameter is chosen from the group comprising the maximal amplitude PI=Imax-Imin of said output signal I(t), where Imax and Imin are respectively the maximum and minimum values of I(t), the required time (TPI) for the output signal I(t) to reach its maximal amplitude PI from the beginning (TL) of the contrast uptake, the mean transit time (MTT) corresponding to the time during which the value I(t) of the output signal is higher than the mean value (Imax+Imin)/2, the area under the curve, the slope of the wash in, the area under the wash in and the area under the wash out are parameters suitable for tumoral vascularization or tumoral angiogenesis detection (ii) comparing said at least one determined parameter with reference parameters
