For example, in a state where the stem cell content is increased, cells are cultured in a temperature range where the hydration power of the polymer is weak on a cell culture support whose surface is coated with a polymer whose hydration power changes in the temperature range of 0 to 80 ° C. It is possible to obtain a cell sheet with high hyaluronic acid-producing ability by culturing and then peeling the cultured cells in a sheet form by changing the culture solution to a temperature at which the polymer has a strong hydration power. Become.例えば、幹細胞含有率を高めた状態で、０～８０℃の温度範囲で水和力が変化するポリマーを表面に被覆した細胞培養支持体上で、ポリマーの水和力が弱い温度域で細胞を培養し、その後、培養液をポリマーの水和力が強い状態となる温度に変化させることで培養した細胞をシート状で剥離させることにより、ヒアルロン酸産生能の高い細胞シートを得ることが可能になる。
