A kind of endoscope system and its light source machine, wherein, endoscope system includes light source machine, introscope and image capture unit, light source machine includes a guiding device and a plurality of arbitrary source mould groups, wherein, light source machine can accurately issue the wave band light source of user's demand by arbitrary source mould group, and by light emitted by guiding device guidance light source module group to introscope. Introscope has a light source input terminal, a test side and an image output end, wherein, light source input terminal is connect with light-emitting window, and to received from light emitted by light-emitting window, and self-test end-fire goes out, when test side being made to detect a target to be measured by the light that light source machine issues, the image data of target to be measured can be accurately obtained, and self imaging output end exports.一種內視鏡系統及其光源機,其中,內視鏡系統包含有光源機、內視鏡以及影像擷取裝置,光源機包括有一導光裝置以及複數個獨立光源模組,其中,光源機藉由獨立光源模組可以精確地發出使用者需求的波段光源,並藉由導光裝置引導光源模組所射出的光線至內視鏡。內視鏡具有一光源輸入端、一檢測端以及一影像輸出端,其中,光源輸入端與出光口連接,並且用以接收自出光口所射出之光線,並自檢測端射出,使檢測端藉由光源機所發出之光線檢測一待測標的時,可以準確取得待測標的的影像資料,並自影像輸出端輸出。