It describes the polypeptides of Hppd and plants containing this substance, which show complete tolerance for one or more Hppd-inhibiting herbicides belonging to various chemical categories. A group of mutant Hppd polypeptides were designed. They have little or no affinity with Hppd and Y herbicides.Meanwhile,The separation rate of HppD inhibitors from Hppd mutant polypeptides increased so that HppD inhibitors no longer act as slow-binding or slow-binding inhibitors.Not like this.They become completely reversible inhibitors Especially,It provides isolated polynucleic acid to encode mutant HPD polypeptide, so that herbicides belonging to different chemical classes of HPD inhibitors are tolerant. In addition,Amino acid sequences corresponding to polynucleotides are provided. 1. Characteristic 1: A recombinant nucleic acid molecule, which encodes a series of amino acids from the polypeptide of 4-hydroxyphenylpyridinic acid dioxylase (Hppd), containing (a) an amino acid in the amino acid position, equivalent to 268 amino acids. SEQ ID No.1,(b) Prolina, which corresponds to the position of amino acid 335 in SEQ 1.(c) The position of amino acid 336 conforming to SEQ number 1 and (d) and a Serina corresponding to amino acid 337 of SEQ number 1, and the Hppd polypeptide can tolerate one or more herbicides； Hppd inhibitors.Se describen polipéptidos de HPPD y las plantas que los contienen que muestran una tolerancia plena contra uno o más herbicidas inhibidores de HPPD que pertenecen a varias clases químicas. Se diseñan un conjunto de polipéptidos de HPPD mutante que no tienen o solamente tienen una afinidad significativamente reducida a los herbicidas inhibidores de HPPD y, al mismo tiempo, la velocidad de disociación de los inhibidores de HPPD del polipéptido de HPPD mutante aumenta en tal medida que los inhibidores de HPPD ya no actúan como inhibidores de unión lenta o de unión lenta y fuerte, sino que en lugar de esto, se convierten en inhibidores completamente reversible
