The invention provides a composition used for preventing mosquito bite, the preparation and the use thereof. The composition comprises (wt.%) organic acid (0.1-9.9) as active agent, and water (88-99.6) as solvent. The organic acid is acetic acid, propionic acid, n-butyric acid, i-butyric acid, n-valeric acid, n-hexylic acid, n- heptanoic acid, L-lactic acid, and/or malic acid. The composition has good effect on preventing mosquito bite by imitating skin odor, breathing odor and interfering olfaction. The raw materials are in terms of food-stage standard, thus composition is safe to human body. The composition has no killing ability to mosquito, no resistance and ecological risk and has simple preparation method, low production cost and no wastes. The composition can be used for preparing mosquito bite preventing product, itching relieving and swelling removing product used after being bitten by mosquito, and product for sterilizing and cleaning air.
