The present invention provides novel, isolated, tumor-associated antigens, andmethods for identifying such antigens in a biological sample, and of screeningfor the presence of such an antigen in a biological specimen, wherein thetumor antigen identified reacts with serum from a subject treated with avaccine comprising a cytokine and proliferation-incompetent tumor cells whichexpress the tumor-associated antigen. Also provided are kits for carrying outthe methods of the invention.