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サバイビンについての細胞解析方法、並びにそれを利用した癌の解析方法および薬効のスクリーニング方法
专利权人:
オリンパス株式会社
发明人:
秋吉 竜太郎
申请号:
JP20130151928
公开号:
JP6223037(B2)
申请日:
2013.07.22
申请国别(地区):
日本
年份:
2017
代理人:
摘要:
PROBLEM TO BE SOLVED: To provide a method for analyzing cells for the intracellular dynamics of survivin protein, and survivin promoter activity.SOLUTION: A method for analyzing cells comprises (1) preparing a first vector comprising a survivin promoter and a gene encoding photoprotein, and a second vector that is an expression vector comprising a promoter, a survivin gene, and a gene encoding fluorescence protein; (2) introducing the first vector and the second vector into a cell; (3) exposing the cell of the (2) to a drug; (4) observing the bright field, measuring the fluorescence intensity, and measuring the luminescence intensity, respectively at at least two points in time within duration over before and after the cell is exposed to the drug; and (5) obtaining information about the cell from the result of the (4).
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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