The present invention discloses a method for manufacturing a highly active plural enzymes product, comprising the steps of: (a) providing plural fermentation bacteria and fruit(s) and/or vegetable(s) jam in a container to form a mixing fermentation liquid； (b) the plural fermentation bacteria is fermented as injecting carbon dioxide, oxygen and nitrogen into the container, in a first fermentation stage； (c) after the first fermentation stage, a second fermentation stage is conducted； and (d) a vacuum ultrasonic and homogenization treatment for the mixed fermentation liquid in the container is performed. During the fermentation, PH value, the proportion of carbon dioxide, oxygen, and nitrogen are adjusted as different stages.本發明揭露一種高活性多種類酵素產品之製作方法，包含以下步驟：(a)提供多種發酵菌種、蔬果於一容器中以形成一混合發酵液；(b)於內含多種發酵菌種之混合發酵液之容器內通入二氧化碳、氧氣與氮氣，於一第一階段中發酵；(c)於第一階段之後，進行一第二階段發酵；以及(d)進行一真空超音波均質處理此容器內之混合發酵液。在發酵期間，PH值、二氧化碳的比例、氧氣的比例、氮氣的比例均隨著不同的階段而有所調整。
