Provided herein are methods of populating a target tissue of interest of a subject with particles of a substance of interest by precipitation. Example methods include administering a solution to a subject, the solution including the substance of interest and a pharmaceutically acceptable solvent. The substance of interest must be soluble in a pharmaceutically acceptable, water-miscible solvent but insoluble in tissue fluids of the target tissue of interest and the pharmaceutically acceptable solvent must be freely miscible with the tissue fluids of the target tissue of interest. According to example embodiments, the solution is administered in a volume of the pharmaceutically acceptable solvent that will be diluted by tissue fluids in the target tissue of interest following administration at a rate that will result in precipitation of an effective amount of particles of the substance of interest in a range of sizes that will accomplish a purpose for which the substance is administered. Examples of the present methods include administering the solution to tissue of an animal, such that the substance of interest in the solution precipitates and provides an effective amount of insoluble particles of the substance of interest to the tissue. Also provided are kits that include the solution or components thereof and methods of making such solutions.
